Thermo Fisher Scientific
Qubit RNA HS assay Kit
Q32852
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Bacterial total RNA for RNA-seq library prep were quantified using Qubit RNA HS assay kit. This assay protocol is more specific than spectrophotometer based quantification. Quantification results are reliable and recommended for further downstream library prep including rRNA removal. Method can detect even picogram levels of RNA in the samples.
For RNA-seq samples preparation
Bacterial Total RNA
Bacterial RNA was diluted to 1:10. This 1:10 diluted samples was used at 1:200 dilution to measure RNA quantity using assay kit with manufacturer's instructions.
Run standards first and dilute origin RNA samples before making actual measurements
1:10 diluted samples with 1:200 dilution (working buffer of Qubit RNA HS assay kit) gave me 450 ng/microliter of Total RNA.
None
Easy to use assay kit and specific to RNA
Always use diluted RNA samples especially RNA from bacterial samples.
Product Name: Qubit RNA HS Assay KitSupplier Page