Bethyl Laboratories
MafA IHC Antibody
IHC-00352
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MafA is an islet enriched transcription factor that is present in ~85% of all islet beta cells. It is specifically expressed in beta cells. We aimed to optimize staining conditions in pancreatic mouse cryosections for MafA.
Immunofluorescence
Mouse pancreas cryosection
1:1000, Overnight, 4 Deg Celcius
5% Normal Donkey Serum in 1%BSA/PBS
1:1000, Donkey anti-Rabbit Cy3
N/A
MafA was detected in nearly all Insulin+ cells in the mouse islets. No cross reaction within alpha cells that specifically express MafB, a close Maf family member of MafA.
None
Excellent detection at 1:1000
Excellent detection of MafA at 1:1000 in mouse cryosections.