Pax6 Staining in Pancreatic Cryosection

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Covance

Product Name:

Purified anti-Pax-6 Antibody

Catalog Number:

PRB-278P

The Pax6 transcription factor is a pan-endocrine marker in the islets of langerhans. We were optimizing staining conditions in pancreatic cryosections, and found a 1:1000 dilution works best.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreas cryosection

Primary Incubation

1:1000, Overnight, 4 Deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-Rabbit Cy3

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

Antibody stained well in nearly all endocrine cells of islets found in the mouse pancreas.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent detection at 1:1000

The Bad

None

The Bottom Line

Excellent detection in cryosections at 1:1000

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