Glut2 Staining in Pancreatic Cryosection

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Glut2 Antibody (C-19)

Catalog Number:

sc-7580

Glut2 is a low-affinity glucose transporter present in pancreatic beta cells, hepatocytes and basolateral membranes of intestinal and epithelial cells. We optimized this antibody in cryosections to detect endogenous Glut2 in mouse pancreas sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreas cryosection

Primary Incubation

1:500, Overnight, 4 Deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-Goat Cy3

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

This antibody detected Glut2 only in Insulin+ beta cells in mouse pancreas cryosections. This worked best at 1:500 dilution

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent detection at 1:500

The Bad

None

The Bottom Line

Excellent detection at 1:500.

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