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PE/Cy7 anti-mouse/human CD11b Antibody
101215
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In order to study immune cell influx during a self limiting inflammatory response in the lung, LPS was instilled into mouse lungs. 24 hours later, brohchoalveolar lavage was performed to isolate airway cells. CD11b antibody was used as a second marker for neutrophils, as well as a phenotypic marker for infiltrating monocytes and alveolar macrophages. This antibody was recommended by a colleague.
Flow Cytometry
Mouse primary immune cells
1:200 in PBS on ice
NA
Flow cytometer
Clearly identified CD11b positive cells
None
Clear separation of positive and negative cells
Super clean antibody. Highly recommended.