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APC anti-mouse CD45
103111
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In order to study immune cell influx in an infection related lung inflammation, LPS was instilled into mouse lungs. 24 hours later, brohchoalveolar lavage was performed to isolate airway cells. CD45 antibody was used to identify hematopoietic cells so that the proportions of infiltrating immune cells can be identified by other antibodies specific for each cell type. This antibody was recommended by a colleague.
Flow Cytometry
Bronchoalveolar lavage cells
1:100 in PBS on ice
NA
Clearly identified CD45 positive cells, allowing for investigation of the infiltrating immune cells.
None
Clean antibody
Very good antibody for identifying CD45 positive cells