APC Anti-Mouse CD45

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD45

Catalog Number:

103111

In order to study immune cell influx in an infection related lung inflammation, LPS was instilled into mouse lungs. 24 hours later, brohchoalveolar lavage was performed to isolate airway cells. CD45 antibody was used to identify hematopoietic cells so that the proportions of infiltrating immune cells can be identified by other antibodies specific for each cell type. This antibody was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bronchoalveolar lavage cells

Primary Incubation

1:100 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

Clearly identified CD45 positive cells, allowing for investigation of the infiltrating immune cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clean antibody

The Bad

None

The Bottom Line

Very good antibody for identifying CD45 positive cells

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