PE Anti-Mouse CD45

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD45

Catalog Number:

103105

Bronchoalveolar lavage was performed on mice treated with LPS (24 hr), and the cells were isolated and stained for flow cytometry. CD45 antibody was used to select hematopoietic cells to further characterize the recruited cell population. This product was chosen because it was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse primary cells

Primary Incubation

1:100 in PBS on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Clearly identified CD45 positive cells, allowing us to determine the proportions of cells that infiltrated the lung.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear result

The Bad

None

The Bottom Line

Highly recommended for mouse primary cells

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