Easy, Reliable ELISA

University of California, San Diego
Department of Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse CCL3/MIP-1 alpha DuoSet ELISA

Catalog Number:

DY450-05

This works well to detect cytokines in lung homogenate (murine samples).

Experimental Design and Results Summary

Application

ELISA

Starting Material

Lung homogenate

Protocol Overview

Mice were infected with M. tuberculosis for 3 weeks. Lungs were collected and homogenized in 5mL PBS-T. 1mL of homogenate was aliquoted and spun down at max speed, and the supernatant was filtered through a 0.2um PVDF filter. Sample was diluted in the appropriate buffer as per product guidelines

Tips

May need to try several dilutions to get your sample within the standard curve.

Results Summary

Great, easy detection of MIP-1a

Additional Notes

None

Image Gallery

Summary

The Good

Works consistently

The Bad

None

The Bottom Line

I like the product and would use it again

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