BioLegend
Mouse IL-1β ELISA Kit deluxe
432604
Info: View Product View Product Specs
Our aim was to monitor IL-1b release from cultured mouse microglia. For this we generated mixed glial cell cultures from neonatal mice (p0-2) in 24-well plates and stimulated the cells for 2h with LPS to induce production of pro-IL-1β in microglia. We subsequently stimulated cells with 1.5mM ATP to induce P2X7-mediated inflammasome formation and cleavage of pro-IL-1β and release of IL-1β into the supernatant. We chose this kit because of good experiences with other kits from BioLegend.
ELISA
None
We stimulated microglia for 2h with LPS to induce production of pro-IL-1β in microglia. We subsequently stimulated cells with 1.5mM ATP to induce P2X7-mediated inflammasome formation and cleavage of pro-IL-1β and release of IL-1β into the supernatant
Change pipette tips during standard dilution for optimal curve
Supernatants contained 100-300pg/ml mouse IL-1β, depending on the number of microglia in the mixed glial cell culture.
Fast and easy to handle
Reliable kit for the detection of mouse IL-1b released from cultured microglia
Product Name: Mouse IL-1ß ELISA MAX™ DeluxeSupplier Page