Phospho-MEK (Ser221) Antibody from Novus Biologicals

March 10, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Phospho-MEK (Ser221) Antibody

Catalog Number:

NB100-82033

This antibody was used to detect phosphorylation of MEK at S221 site in human breast cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody detected phosphorylated MEK at S221 site by western blot.

Additional Notes

None

Related Categories

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Summary

The Good

Detects specific band easily

The Bad

Has some non-specific bands

The Bottom Line

This is a good antibody to detect phosphorylated MEK in breast cancer cells.

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