CPSF160 Antibody from Bethyl Laboratories

Biological Sciences
Columbia University
Postdoc

Overall

Quality of Results

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Company:

Bethyl Laboratories

Product Name:

CPSF160 Antibody

Catalog Number:

A301-580A

I am investigating functions of RNA 3'processing complexes. The goal of this experiment was to test the specificity of the antibody to detect CPSF160 expression in HeLa cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa Whole Cell Lysates

Primary Incubation

1:500 dilution overnight in 5% milk buffer at 4C

Blocking Agent

5% milk in TBS

Secondary Incubation

1:10000 anti-rabbit secondary at RT for 1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

Multiple bands appeared in addition to the band with the preditcted MW, possibly because of post-translational modifications

Additional Notes

None

Related Categories

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Summary

The Good

CPSF160 band(s) can be obtained

The Bad

Higher dilution rate may be required

The Bottom Line

This antibody can detect CPSF160

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