Bethyl Laboratories
CPSF30 Antibody
A301-585A
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I am investigating functions of RNA 3'processing complexes. The goal of this experiment was to test the specificity of the antibody to detect CPSF30 expression in HeLa cells.
Western Blot
HeLa Whole Cell Lysates
1:2000 dilution overnight in 5% milk buffer at 4C
5% milk in TBS
1:10000 anti-rabbit secondary at RT for 1 hour
NA
ECL
Two isoforms of CPSF30 bands were efficiently detected
Lower dilution may also work for this antibody
Specific and strong bands can be obtained
None
Excellent antibody to detect two different isoforms of CPSF30 protein