CSTF77 Antibody from Bethyl Laboratories

Biological Sciences
Columbia University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bethyl Laboratories

Product Name:

CSTF77 Antibody

Catalog Number:

A301-096A

I am investigating functions of RNA 3'processing complexes. The goal of this experiment was to test the specificity of the antibody to detect CSTF77 expression in HeLa cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa Whole Cell Lysates

Primary Incubation

1:2000 dilution overnight in 5% milk buffer at 4C

Blocking Agent

5% milk in TBS

Secondary Incubation

1:10000 anti-rabbit secondary at RT for 1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

The results show a single band at the correct molecular weight.

Additional Notes

Lower dilution may also work for this antibody

Related Categories

Image Gallery

Summary

The Good

Single and strong band can be obtained

The Bad

None

The Bottom Line

Excellent antibody to detect CSTF77 protein

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