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CD40 alexafluor488
102910
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A spleen was harvested from a C57BL/6 mouse and a single cell suspension was made. The cells were stained with the antibody that I am reviewing and also stained with an anti-mouse CD11c antibody. For data analysis, I gated on the CD11c positive population and assessed expression levels on a histogram for the antibody that I am reviewing. This antibody was used to assess CD40 expression on dendritic cells from mouse splenocytes.
Flow Cytometry
Mouse splenocytes
20 min on ice using 1x10^6 cells/100ul
Fc block was used
NA
Flow cytometry
The population stained with CD40 antibody is distinguishable from the negative population. Titration of antibody 5 fold showed that the percentage of CD40+ doesn't change and there is a slight shift in the MFI.
None
Bright
Good antibody for measuring CD40 expression.