ImmunoStar, Inc.
Vesicular Monoamine Transporter 2 Rabbit Antibody
20042
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Vesicular Monoamine Transporter 2 is a key phenotype of dopaminergic neurons. This antibody was used to confirm if striatal TH interneurons are dopaminergic neurons.
Immunofluorescence
Adult mouse brain
1:2000 for 60-70 hours @ 4ºC
10% Normal serum + 3% bovine serum albumin (BSA) + 0.5% Triton X in PBS for 4-6hr @ RT
1:400 Donkey Anti Rabbit IgG with Alexa 594 for 4-6hr @ RT
NA
Fluorescence
Mouse Striatal and substantia Nigra tissue was process for fluorescence microscopy. Works very well. The staining was also done in transgenic mice that express GFP on TH promoter for a positive control.See Xenias, et al. "Are striatal tyrosine hydroxylase interneurons dopaminergic?." The Journal of Neuroscience 35.16 (2015): 6584-6599.
Tried different concentration and 1:2000 works the best.
Best marker to test if neuron expresses VMAT2
None
Excellent Antibody!!!!