CD62L Alexa Fluor 647 Antibody

cardiovascular disease
Yale
postdoc associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD62L Alexa Fluor 647

Catalog Number:

104421

The general aim of my research is to investigate how lipids regulate immune cell function. The specific goal of this experiment is to determine the percentage of naive and activated T cells. BioLegend products are chosen for this experiment because the antibodies from this company usually work very well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T cells

Primary Incubation

Dilution factor is 100; 30 minutes on ice in PBS+0.1%FCS.

Blocking Agent

FcR blocker.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS

Results Summary

I use this antibody to study the percentage of naive T cells. The separation is clear and background noise is very low.

Additional Notes

None

Related Categories

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Summary

The Good

This antibody can label the T cells very clearly, with very low background noise.

The Bad

Not found so far.

The Bottom Line

I think this antibody works very well for surface staining of mouse T cells.

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