Good Antibody to Detect Endothelial Cells

IBIMER
CIBM
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD31 (PECAM-1) Biotin

Catalog Number:

13-0311-81

Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the presence of vessels in a PP section.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse PP section

Primary Incubation

1ul/100ul; 1 hour at RT

Blocking Agent

10% goat serum

Secondary Incubation

Alexa Fluor 647 Anti-rat (1:500)

Tertiary Incubation

NA

Detection

Leica confocal microscope

Results Summary

You can see a nice staining of endothelial cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Highly recommended antibody

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