Good Antibody to Detect Follicular Dendritic Cells

February 09, 2016

IBIMER
CIBM
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-Mouse CD21/CD35 Biotin

Catalog Number:

13-0211-81

Our group is interested in murine secondary lymphoid organs, such as lymph nodes (LN) or Peyer´s patches (PP). Here, we analyzed the presence of Follicular Dendritic cells in in a LN section.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymph node

Primary Incubation

1ul/100ul; 1 hour at RT

Blocking Agent

10% goat serum

Secondary Incubation

AlexaFluor 647 Streptavidin: 1/500, 30min, RT

Tertiary Incubation

NA

Detection

Leica confocal microscope

Results Summary

You can see a nice staining of follicular dendritic cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Highly recommended antibody

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