HIF-2 alpha Antibody from Novus Biologicals

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

HIF-2 alpha Antibody

Catalog Number:

NB100-122

This antibody was used to detect HIF-2 alpha expression in breast cancer cell lines in response to DMOG treatment by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody detects HIF-2 alpha expression in different breast cancer cell lines in response to DMOG treatment.

Additional Notes

None

Related Categories

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Summary

The Good

Detects specific bands easily

The Bad

Signal becomes weak a short period after dilution

The Bottom Line

This antibody is good to detect HIF-2 alpha expression in breast cancer cell lines, but should be used as fresh as possible.

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