Good Marker for Sorting Naive CD4 T Cells

February 16, 2016

Research Participation Program
Oak Ridge Institute for Science and Education
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD4

Catalog Number:

100437

We study T cell responses to antigen presenting cells. This is an excellent antibody for gating populations of CD4+ T cells in murine spleens.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Live murine splenocytes, red blood cells lysed

Primary Incubation

1:100 1 ul Brilliant Violet 421™ anti-mouse CD4 on ice for 10 minutes

Blocking Agent

1x PBS + 2% FBS + 2mM EDTA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

Strong, specific staining of CD4+ T cells.

Additional Notes

None

Related Categories

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Summary

The Good

It was easy to identify the CD4+ T cells in total murine splenocytes.

The Bad

No drawbacks observed.

The Bottom Line

I would recommend this useful and bright antibody to any immunology lab.

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