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Brilliant Violet 421™ anti-mouse F4/80
123132
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We are interested in studying macrophage activation phenotypes in mice. We collect peritoneal exudate cells, and use flow cytometry to gate out the non-macrophage population. This antibody provides a bright signal on a channel that was useful for our panel.
Flow Cytometry
Mouse peritoneal exudate cells
1:100 BV421 anti-mouse F4/80 + 1:100 FITC anti-mouse CD19 on ice for 20 minutes
1x PBS + 2% FBS + 2mM EDTA
NA
BD LSR Fortessa
Clearly separates double negative, CD19+F4/80-, and CD19-F4/80+ populations.
None
Clear, consistent results.
No drawbacks observed.
The bright signal from this antibody is a useful tool in the study of murine peritoneal macrophages.