Easy to Use Lentivirus Packaging System

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

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Company:

Clontech

Product Name:

Lenti-X Packaging Single Shots

Catalog Number:

631275

This product is an easy to use packaging system for lentiviruses. It can be used with 3rd generation compatible pro-viral vectors and you just add your plasmid diluted in water to the single shot tube and then transfect the cells.

Experimental Design and Results Summary

Application

Lentivirus Production

Starting Material

None

Protocol Overview

Add pro-viral plasmid in water to single shot tube. Mix and incubate for 10 minutes. Transfect HEK.

Tips

Add mixture dropwise

Results Summary

80+% transfection efficiency is seen with proviral vectors using this system. Viruses packaged with this system are infectious and will transduce cells in downstream application.

Additional Notes

None

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Summary

The Good

Ease of use

The Bad

Much more expensive than the alternative

The Bottom Line

Easy to use packaging system if you have the money.

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