Detection of T Cells Using Anti-CD3

BBM III e Inmunologia
Universtity of Granada
Post-doc

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Quality of Results

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Company:

BioLegend

Product Name:

FITC anti-human CD3 Antibody

Catalog Number:

317305

Our work involves the characterization of human fibroblasts from different tissues and their relation with leukocytes. In this case, we analyzed the proportion of B and T cells in human tonsils.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tonsil cells

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACScalibur

Results Summary

Gating on forward/side scatter alone gives a uniform T cell population with respect to CD3.

Additional Notes

None

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Summary

The Good

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The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

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