Nice Antibody to Detect CD45+ Cells from the Tonsil

February 24, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP anti-human CD45 Antibody

Catalog Number:

368505

Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CD45 expression by human cells from the tonsil and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tonsil cells

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACScalibur

Results Summary

Gating on forward/side scatter alone gives a uniform population with respect to CD45. CD45 is shown in red and isotype control is shown in blue

Additional Notes

None

Related Categories

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Summary

The Good

No background

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

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