R&D Systems
Human/Mouse CXCL12/SDF‑1 Antibody
MAB350-SP
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Browse Similar Products: CXCL12 Flow Cytometry Antibodies
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the CXCL12 expression by human fibroblasts and we found this Ab really useful.
Flow Cytometry
Human fibroblast
1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT
1% BSA + 4% human serum
NA
FACScalibur
Gating on forward/side scatter alone gives a uniform population with respect to CXCL12. CXCL12is shown in blue and isotype control is shown in red
Cells were fixed in 4%PFA for 30 min at 4ºC and washed with 0,5% saponine in PBS
No background
None
It is an easy to use antibody that delivers good results