Nestin Expression by Human Fibroblasts

February 19, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human Nestin Antibody

Catalog Number:

MAB1259-SP

Our work involves the characterization of human fibroblasts from different tissues. We analyzed Nestin expression by human fibroblasts and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human fibroblasts

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACScalibur

Results Summary

Gating on forward/side scatter alone gives a uniform population with respect to Nestin. Nestin is shown in blue and isotype control is shown in red.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

It is an easy to use this antibody that delivers good results

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