STRO-1 Expression by Human Fibroblasts

February 08, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human STRO‑1 Antibody

Catalog Number:

MAB1038

Our work involves the characterization of human fibroblasts from different tissues. We analyzed the STRO-1 expression by human fibroblasts and we found this Ab really useful.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human fibroblasts

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

FITC Anti-mouse IgM (1:500)

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Gating on forward/side scatter alone gives a uniform population with respect to STRO-1. STRO-1 is shown in blue and isotype control is shown in black.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Background

The Bottom Line

It is an easy to use antibody that delivers good results

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