IgM Antibody Review

February 03, 2016

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

IgM FITC Antibody

Catalog Number:

406505

Mice are latently or lytically infected with virus. Splenocytes are collected and RBC lysed before staining and fixing for flow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 10 minutes, Ice

Blocking Agent

Fc Block

Secondary Incubation

1:200; 25 minutes, Ice

Tertiary Incubation

NA

Detection

LSRII

Results Summary

Stained for various immune cell types.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use

The Bad

May vary with experimental set up.

The Bottom Line

Good antibody. So far easy to use compared to other IgM antibodies we have tried.

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