Novus
Thioredoxin antibody
NBP1-31090
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Browse Similar Products: Thioredoxin Western Blot Antibodies
I selected the thioredoxin antibody from Novus, NBP1-31090, because the western blot on their website looked beautiful and because I thought Novus had a good reputation for other antibodies. I tried the antibody and got nothing except for high-up bands much too high to be real thioredoxin. I contacted tech support and they sent me a replacement vial. I tried this one too, and got only a very faint band at the right MW, still with a very bright band much too high up. I compared this to an identical sample incubated with ProteinTech's TRX antibody, and found that the ProteinTech antibody gave me an enormously bright band with the same amount of sample.
Western Blot
Hela lysate
1:1000, overnight, 4 deg C
Odyssey Blocking Buffer
1:20,000, 1 hr, room temp
NA
LiCor Odyssey Scan
I got a band of the wrong molecular weight and little to no band at the right molecular weight when I used the Novus TRX antibody.
None
They gave me a refund
It didn't work
Don't buy it till they make a new batch