Immunofluorescence of Glucagon and Insulin in Pancreatic Mouse Cryosection

February 05, 2016

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Glucagon Antibody

Catalog Number:

NBP1-67575

We aimed to optimize Glucagon staining in our pancreatic mouse sections using a Rabbit Glucagon antibody. Novus produces excellent antibodies therefore we purchased and optimized from them.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreatic cryo-section

Primary Incubation

1:1000, 4 Deg C, overnight.

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS (w/v)

Secondary Incubation

1:1000. rabbit IgG-Cy2. 2 hours, Room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

This glucagon antibody worked superb in our mouse pancreatic cryosections at 1:1000.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent detection at 1:1000

The Bad

None

The Bottom Line

Excellent detection at 1:1000. No evidence of cross-reactions with other hormone positive cells within the islet.

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