Sigma-Aldrich
Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody produced in mouse
F3777-.2ML
Browse Similar Products: Actin Flow Cytometry Antibodies
Our work involves the characterization of human fibroblasts from different tissues. We analyzed the ASMA expression by human fibroblasts and we found this Ab really useful.
Flow Cytometry
Human fibroblast
1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT
1% BSA + 4% human serum
NA
FACS Calibur
Gating on forward/side scatter alone gives a uniform population with respect to ASMA. ASMA is showed in blue and isotype control is showed in red
Cells were fixed in 4%PFA for 30 min at 4ºC and washed with 0,5% saponine in PBS
No background
None
It is an easy to use antibody that delivers good results