Phospho-4E-BP1 (Thr70) Antibody from Cell Signaling

February 19, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

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Company:

Cell Signaling Technology

Product Name:

Phospho-4E-BP1 (Thr70) Antibody

Catalog Number:

9455

This antibody was used to detect expression of phospho-4EBP1 (Thr70) in response to rapamycin treatment in MDA-MB-231 human breast cancer cells by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:1000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

NA

Detection

ECL Prime

Results Summary

This antibody detected the expression of phospho-4EBP1 at T70 site in MDA-MB-231 cells.

Additional Notes

None

Related Categories

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Summary

The Good

Detects specific band with clean background

The Bad

Signal is a little weak.

The Bottom Line

This is a good antibody to detect phospho-4EBP1 at T70 site by Western Blot.

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