BD Biosciences
Anti-Rat Ig, κ/Negative Control Compensation Particles Set
552844
Compensation Bead Kits have been developed for optimal compensation of fluorescence spillover of fluorochrome-conjugated antibodies. This will help to separate the positive and negative population during flow cytometric analysis involving multiple antibodies.
Flow cytometry
BD™ CompBeads Anti-Rat Ig, κ
1. Label the number of tubes required for the assay, one for each antibody to be used in the assay and one tube for unstained control. 2. Add 2 drops of the positive beads and 2 drops of the negative beads. 3. Spin down the tubes and resuspend in 50ul of FACS buffer. 4. To this 50ul, add only 0.5ul of the specific antibody you wish to compensate in the assay. 5. Leave one tube as unstained control. 6. Leave for at least 15 mins in the dark. 7. Wash the tube and calculate compensation using these beads.
Knowledege of flow cytometry required
Was able to accurately distinguish between positive and negatively stained populations.
None
Fast and accurate compensation
Very useful for multicolor flow cytometry