Phospho-EGF Receptor (Tyr1068) Antibody from Cell Signaling

March 01, 2016

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-EGF Receptor (Tyr1068) Antibody

Catalog Number:

2234S

This antibody can be used to detect the expression of phosphorylated EGF receptor at Y1068 site by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cancer cells whole lysates

Primary Incubation

1:2000, overnight, 4 degrees

Blocking Agent

Non-fat milk

Secondary Incubation

1:2000, 1 hour, RT

Tertiary Incubation

None

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody can be used to detect phosphorylated EGFR at Y1068 site by western blot.

Additional Notes

None

Related Categories

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Summary

The Good

Signal is strong and specific.

The Bad

None

The Bottom Line

This is a perfect antibody for the detection of phosphorylated EGFR at Y1068 site by western blot.

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