Anti-Mouse CCR6 PerCp-Cy5.5

Internal Medicine.
Rush University Medical Center
Research Assistant

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse CD196 (CCR6)

Catalog Number:

129809

In order to find out what cells play a roll in the proteoglycan (PG)-induced arthritis (PGIA) model, we identify the cell populations in the different tissue of PGIA mice by flow cyotemetry. For this experiment, 1X10^6 splenocytes or peritoneal cells were treated with PMA(25ng/ml) and Ionomycin (500ng/ml) at 37oC for 4 hours. Then cells were harvested and stained with 0.5ul PerCP-Cy5.5 anti-mouse CCR6 and other Abs in 50ul FACS buffer for 30 min at 4oC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes and Peritoneal cells

Primary Incubation

Cells stained with 0.5ul PerCP-Cy5.5 anti-mouse CCR6 and other Abs in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

Cells were blocked with 0.5ul CD16/CD32 for 10 min at 4oC

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

CCR6 expression increased after treatment with PMA and ionomycin.

Additional Notes

None

Related Categories

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Summary

The Good

Good antibody

The Bad

None

The Bottom Line

Good antibody

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