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PerCP/Cy5.5 anti-mouse CD196 (CCR6)
129809
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In order to find out what cells play a roll in the proteoglycan (PG)-induced arthritis (PGIA) model, we identify the cell populations in the different tissue of PGIA mice by flow cyotemetry. For this experiment, 1X10^6 splenocytes or peritoneal cells were treated with PMA(25ng/ml) and Ionomycin (500ng/ml) at 37oC for 4 hours. Then cells were harvested and stained with 0.5ul PerCP-Cy5.5 anti-mouse CCR6 and other Abs in 50ul FACS buffer for 30 min at 4oC.
Flow Cytometry
Splenocytes and Peritoneal cells
Cells stained with 0.5ul PerCP-Cy5.5 anti-mouse CCR6 and other Abs in 50ul FACS buffer for 30 min at 4oC.
Cells were blocked with 0.5ul CD16/CD32 for 10 min at 4oC
NA
CCR6 expression increased after treatment with PMA and ionomycin.
None
Good antibody