Santa Cruz Biotechnologies, Inc.
MOZ Antibody (N-19): sc-5713
sc-5713
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Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use western blots and subsequent sequencing to screen potential knockout clones. We chose this antibody, because we've had some luck with Santa Cruz antibodies before.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk in PBST
1:3000 antigoat secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from BioRad
This was the worst antibody I have used this year. Very high background and weak non-specific staining made the data unreadable. I ran the WB multiple times with fresh reagents, thinking the background was due to a bad reagent. However, every WB looked like this one. This seems to be a pattern with Santa Cruz antibodies made in goat specifically.
None
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High background, non-specific staining
Would not recommend.