Santa Cruz Biotechnologies, Inc.
YEATS2 Antibody (N-16)
sc-103328
Info: View Product View Product Specs
Browse Similar Products: YEATS2 Western Blot Antibodies
Our lab generates a lot of knockouts using Crispr-cas9 technology. We use Western blots and subsequent sequencing to screen the knockout clones. We chose this antibody, because Santa Cruz generally produces good antibodies in our experience.
Western Blot
Whole cell lysates from mouse ES cells
1:500 dilution overnight in 5% milk buffer at 4C
5% milk
1:3000 antirabbit secondary at RT for 1 hour
NA
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
There are multiple bands near the 150 kda mark, which makes the WB results hard to interpret. We believe the top band is the specific band, although it is very weak and experiences interference from the much stronger, non-specific band right below it when the membrane is imaged. Not the best antibody for Yeats2.
None
Santa Cruz has a easy refund process
Strong non-specific band
Not the worst antibody but definitely not the best either.