Santa Cruz Biotechnology
PLC beta3 antibody (C-20)
sc-403
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Aortic smooth muscle cell migration into tunica intima is a major cause of restenosis. We have tested various signaling molecules levels in vascular smooth muscle cells to understand the mechanism of their migration. Here, we tested the time-course effect of a chemokine on expression of PLC beta3 in human aortic smooth muscle cells.
Western Blot
Aortic Smooth Muscle Cell lysates
5% skim milk, 1 hr at room temperature
5% skim milk
1:500 dilution/ over night / 4 deg C
Goat anti-rabbit, 1:5000, room temperature for 1 hr
Horseradish peroxidase (HRP)
Time-course effect of chemokine has no effect on PLC beta3 expression
None
Easy to optimize
Need high concentration & gives non-specific bands.
Antibody gives non-specific background.