Bio Rad S3 Cell Sorter Instrument

Kansas State University
Veterinary Diagnostic Laboratory
RA

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

S3 cell sorter

Catalog Number:

S3

The S3 cell sorter is a benchtop cell sorter equipped with two lasers and up to four fluorescence detectors plus forward and side scatter (FSC and SSC). Cells are analyzed using traditional jet-in-air technology. Samples can be sorted at high speeds while maintaining sensitivity and unmatched purity. The S3 cell sorter is also quite small.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Cells in suspension.

Protocol Overview

Cells to be sorted are usually brought in some sort of suspension alone with the material it needs to be sorted into. For example: media.

Tips

Have as many cells as possible to get a good sort.

Results Summary

The sorting process is very easy once the instrument is calibrated and the drop delay is calculated. Experiment setup is fairly easy as well.

Features Summary

Has automated drop delay calculation, stream-to-laser alignment, droplet break-off monitoring, and feedback to ensure sort accuracy.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use.

The Bad

Drop delay can be tricky

The Bottom Line

A user-friendly cell sorter.

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