GAPDH Antibody for Western Blotting

Pharmaceutical Sciences
Northeastern University, Boston
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-GAPDH antibody [6C5]

Catalog Number:

ab8245

I am interested in studying the role of various transcription factors during regenerative processes of the lung and intestine after chemical induced tissue injury. The level of transcription factors during these processes are detected by Western Blotting and this antibody (GAPDH) was used as a loading control. This product was selected because of a high rating from the research community.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung tissue whole cell lysate

Primary Incubation

1:5000 dilution; 1 hr at room temperature

Blocking Agent

5% Milk in TBS-T buffer

Secondary Incubation

1:10000 dilution; 1 hr at room temperature

Tertiary Incubation

NA

Detection

ECL

Results Summary

This antibody gives a specific signal at 36 kDa

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific signal

The Bad

None

The Bottom Line

A nice loading control antibody for Western Blotting

Share your experience with other scientists. Write a Review! »

Join the discussion