Alpha SMA Antibody

Environ. Med.
University of Rochester
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma-Aldrich

Product Name:

Monoclonal Anti-a Smooth Muscle Actin Clone 1A4

Catalog Number:

A 2547

Browse Similar Products: Alpha SMA Immunofluorescence Antibodies

We have used this aSMA antibody for checking TGF beta and oxidant induced alpha smooth muscle actin production in human lung fibroblast cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human fibroblast cells

Primary Incubation

1:500 dilution in 10% Goat serum

Blocking Agent

10% Goat serum for 1 hr at RT

Secondary Incubation

1:1000 in 10% goat serum at RT

Tertiary Incubation

NA

Detection

Imaging using fluorescence microscope

Results Summary

This antibody works great for cells in culture. Shows oxidant/TGF beta induced activation of alpha smooth muscle actin.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works very well in cells

The Bad

Noted none

The Bottom Line

Antibody works well for immunofluorescence and WB in cells

Share your experience with other scientists. Write a Review! »

Join the discussion