BioLegend
Alex 647 Ly5.2
109817
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The aim of my research is to determine the antigen-specific T cell responses during viral infection. This experiment is to determine the percentage of Ly5.2 T cells in an adoptive transfer experiment. This antibody has been chosen because of the good reputation of BioLegend products.
Flow Cytometry
Mouse spleen cells
1:100 dilution; on ice for 30 minutes.
Homemade 2.4G2 blocker.
NA
FACS
This antibody gives a very clear separation between positive and negative populations.
None
Separation is very clear.
This antibody works very well in my hands.