PerCP/cy5.5 CD8 Antibody

January 26, 2016

Immunobiology
Yale University
Postdoc fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Percp/cy5.5 CD8

Catalog Number:

100733

The aim of my research is to determine the antigen-specific T cell responses during viral infection. This experiment is to determine the percentage of CD8 T cells in an adoptive transfer experiment. This antibody has been chosen because of the good reputation of BioLegend products.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen cells

Primary Incubation

1:100 dilution; on ice for 30 minutes.

Blocking Agent

Homemade 2.4G2 blocker.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS

Results Summary

This antibody gives a very clear separation between positive and negative populations.

Additional Notes

None

Related Categories

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Summary

The Good

Separation is very clear.

The Bad

None

The Bottom Line

This antibody works very well in my hands.

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