PCR Purification Kit

U of Miami
Biology
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

GeneJet PCR Purification Kit

Catalog Number:

K0701

This kit is used to purify PCR products and remove primers and extra nucleotides in the PCR solution. This is a quick and easy to use kit.

Experimental Design and Results Summary

Application

PCR Purification

Starting Material

None

Protocol Overview

Amplify PCR product. Follow kit instructions for removing primers and single nucleotides from the PCR product.

Tips

Lower elution volume for higher concentrations

Results Summary

PCR product purified with this kit has been used for downstream applications e.g. cloning successfully. However, the 260/230 readings are sometimes low in PCR products purified by this kit. However, doesn't seem to have affected using with downstream applications.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use

The Bad

260/230 is low occasionally

The Bottom Line

This is a quick kit to clean up PCR products.

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