Checking for Non-Viable Cells

Neurosurgery
BWH/HMS
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

7-AAD

Catalog Number:

559925

Browse Similar Products: 7-AAD Flow Cytometry Antibodies

Working with viruses, it is always useful to check cell viability. Instead of doing a separate experiment every time, it is really convenient to add a cell death marker in the Ab panel and do flow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

7-AAD

Primary Incubation

10 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

650 nm

Results Summary

Data are clean with a nice population or new peak of positive cells - depends if you prefer a counter plot or an histogram. Fast and efficient Ab - 5ul for 10 min at RT. No needs to wash it. I normally stain my sample (human cancer cells) after my extracellular staining, just before going for flow.

Additional Notes

Is not intracellular.

Related Categories

Image Gallery

Summary

The Good

Fast staining

The Bad

Can overlap with FITC and PE

The Bottom Line

It is a convient product: easy to use, fast and not so expensive. Recommended.

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