Cell Signaling Technology
Phospho-PKCdelta (Thr505) antibody
9374
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We tested the time-course effect of a chemokine on phosphorylation of PKC delata in human aortic smooth muscle cells.
Western Blot
Aortic Smooth Muscle Cell lysates
5% BSA, 1 hr at room temperature
5% BSA
1:500 dilution/ over night / 4 deg C
Goat anti-rabbit, 1:5000, room temperature for 1 hr
Horseradish peroxidase (HRP)
We found that chemokine induces the phosphorylation of PKCdelta in human aortic smooth muscle cells in a time-dependent manner.
None
The antibody is very good and it gives a single non-specific band.
Needs high concentration
Antibody is specific and gives mostly a single non-specific band in human aortic smooth muscle cells.