CD31 Antibody to Identify Mouse Skin Blood Vessels

January 27, 2016

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Purified Rat Anti-mouse CD31

Catalog Number:

553370

The goal is to examine the blood vessels in mouse skin wounds. This antibody works exceptionally well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

1/1600 dilution, 45min RT

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti rat Alexa Fluor 594

Tertiary Incubation

None

Detection

Alexa Fluor 594

Results Summary

Perfect staining

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Highly sensitive, strong signal and easy to use

The Bad

None

The Bottom Line

Highly recommended

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