Canine Immunophenotyping - Isotype Control

Veterinary Diagnostic Laboratory
Kansas State University
RA

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Company:

BioLegend

Product Name:

PE/Cy7 Mouse IgG1, κ Isotype Ctrl

Catalog Number:

400125

The purpose was detection of B cell/T cell lymphoma using flow cytometry. This isotope served as a good negative control for distinguishing cells that were taking up positive stain from other antibodies used.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood in EDTA

Primary Incubation

1ul of the isotope for 30mins in the dark at 4 degrees.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS Calibur

Results Summary

Serves as a good isotype control.

Additional Notes

None

Image Gallery

Summary

The Good

Serves the purpose of this experiment

The Bad

NA

The Bottom Line

Good isotype control.

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