Very Good Phoshpo GSK3 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-GSK-3α/β (Ser21/9)

Catalog Number:

9331

I am investigating changes of phospho-GSK3 between different different cancerous cell lines after diverse stress inductions. I am using this antibody since I am also using the total GSK3 antibody for total GSK3 in these cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

RT, 1h, 5% milk in TBST

Secondary Incubation

RT, 1h, 1:5000 5% milk in TBST

Tertiary Incubation

NA

Detection

Bio-Rad clarity

Results Summary

Very good phospho GSK3 antibody

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Perfect phoshp-GSK3 antibody

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