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APC/Cy7 anti-human CD27 Antibody
302816
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The aim of this study was to evaluate memory phenotypes of human peripheral blood T cells. We chose this antibody because it promised to provided reliable staining of CD27+ T cells.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated with the antibody for 30 minutes in the dark at RT. Cells were lysed with BD lysing solution following the instructions on the solution. Then cells were washed with PBS 3 times and then diluted in 300ul of FACS buffer for direct usage in flow cytometry.
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Flow cytometry
This antibody provides good staining of CD27+ T cells and can be used to evaluate the memory phenotype of human T cells.
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Clear identification of CD27+ T cells
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CD27+ T cells can be clearly identified with this antibody using minimal amounts of sample. This helps to evaluate the memory status of human T cells.